Vitamin B12 Reconstitution Calculator
The numbers below are pre-computed for a 30 mg vial of Vitamin B12. Doses reported in the literature and in research protocols for Vitamin B12 sit around 100–1000 mcg (label), intramuscular. Pep-Tidy does the arithmetic only — it is not medical or dosing advice.
How much water to add to a 30 mg Vitamin B12 vial
| Bacteriostatic water | Concentration | Per U-100 unit | Units for 1 mg |
|---|---|---|---|
| 1 mL | 30 mg/mL | 300 mcg | 3.3 units |
| 2 mL | 15 mg/mL | 150 mcg | 6.7 units |
| 3 mL | 10 mg/mL | 100 mcg | 10 units |
| 5 mL | 6 mg/mL | 60 mcg | 16.7 units |
Vitamin B12 units to draw at 30 mL
Reading a 30 mg vial reconstituted with 30 mL of bacteriostatic water on a U-100 insulin syringe.
| Dose | Volume | U-100 units | Doses per vial |
|---|---|---|---|
| 500 mcg | 0.5 mL | 50 units | 60 |
| 1 mg | 1 mL | 100 units | 30 |
| 1.5 mg | 1.5 mL | over syringe capacity | 20 |
| 2 mg | 2 mL | over syringe capacity | 15 |
- 30 mL is a lot of water for a single vial — double-check the number; more than 10 mL usually won't fit in one vial.
- 100 units is a large draw — that usually means the solution is very dilute. Double-check the water amount.
Mixing and storage notes
- Aim the water down the inside wall of the vial, never straight onto the powder, and let it dissolve without shaking.
- Keep lyophilized vials at 2–8 °C and out of light; reconstituted vials are refrigerated and commonly discarded after about 28 days.
- Full walkthrough: how to reconstitute peptides step by step.
Vitamin B12 reconstitution questions
How do you reconstitute Vitamin B12?
A common starting point for a 30 mg vial of Vitamin B12 is 30 mL of bacteriostatic water, which gives 1 mg per mL. Aim the water down the inside wall of the vial, let it dissolve on its own, and roll gently instead of shaking. Pep-Tidy's reconstitution calculator will recalculate the numbers for any other vial size or water volume you use.
How many units of Vitamin B12 is one dose?
At 1 mg per mL, that works out to about 100 units on a U-100 insulin syringe for a 1000 mcg dose. Change the vial size, water volume, or target dose in the calculator and the syringe reading updates with it. This is arithmetic, not a dosing recommendation.
What dosing schedule is reported for Vitamin B12?
Published and community sources describe 100–1000 mcg (label), intramuscular, once weekly, typically run 12 weeks on. These figures describe what has been reported, not what anyone should take — talk to a licensed clinician first.
How should Vitamin B12 be stored?
Lyophilized Vitamin B12 vials are generally kept at 2–8 °C and out of light. Once reconstituted with bacteriostatic water they are refrigerated and commonly discarded after about 28 days. Never re-freeze a reconstituted vial and never shake it.