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TB-500 Reconstitution Calculator

The numbers below are pre-computed for a 5 mg vial of TB-500. Doses reported in the literature and in research protocols for TB-500 sit around 2–2.5 mg loading, 2.5–5 mg maintenance, subcutaneous. Pep-Tidy does the arithmetic only — it is not medical or dosing advice.

How much water to add to a 5 mg TB-500 vial

Bacteriostatic waterConcentrationPer U-100 unitUnits for 2.5 mg
1 mL5 mg/mL50 mcg50 units
2 mL2.5 mg/mL25 mcg100 units
3 mL1.67 mg/mL16.7 mcgover syringe capacity
5 mL1 mg/mL10 mcgover syringe capacity

TB-500 units to draw at 2 mL

Reading a 5 mg vial reconstituted with 2 mL of bacteriostatic water on a U-100 insulin syringe.

DoseVolumeU-100 unitsDoses per vial
1.25 mg0.5 mL50 units4
2.5 mg1 mL100 units2
3.75 mg1.5 mLover syringe capacity1
5 mg2 mLover syringe capacity1

Mixing and storage notes

  • Aim the water down the inside wall of the vial, never straight onto the powder, and let it dissolve without shaking.
  • Keep lyophilized vials at 2–8 °C and out of light; reconstituted vials are refrigerated and commonly discarded after about 28 days.
  • Full walkthrough: how to reconstitute peptides step by step.

TB-500 reconstitution questions

How do you reconstitute TB-500?

A common starting point for a 5 mg vial of TB-500 is 2 mL of bacteriostatic water, which gives 2.5 mg per mL. Aim the water down the inside wall of the vial, let it dissolve on its own, and roll gently instead of shaking. Pep-Tidy's reconstitution calculator will recalculate the numbers for any other vial size or water volume you use.

How many units of TB-500 is one dose?

At 2.5 mg per mL, that works out to about 100 units on a U-100 insulin syringe for a 2500 mcg dose. Change the vial size, water volume, or target dose in the calculator and the syringe reading updates with it. This is arithmetic, not a dosing recommendation.

What dosing schedule is reported for TB-500?

Published and community sources describe 2–2.5 mg loading, 2.5–5 mg maintenance, subcutaneous, twice weekly, typically run 6 weeks on and 2 weeks off. These figures describe what has been reported, not what anyone should take — talk to a licensed clinician first.

How should TB-500 be stored?

Lyophilized TB-500 vials are generally kept at 2–8 °C and out of light. Once reconstituted with bacteriostatic water they are refrigerated and commonly discarded after about 28 days. Never re-freeze a reconstituted vial and never shake it.