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SS-31 Reconstitution Calculator

The numbers below are pre-computed for a 50 mg vial of SS-31. Doses reported in the literature and in research protocols for SS-31 sit around 40 mg (trial dose), subcutaneous. Pep-Tidy does the arithmetic only — it is not medical or dosing advice.

How much water to add to a 50 mg SS-31 vial

Bacteriostatic waterConcentrationPer U-100 unitUnits for 40 mg
1 mL50 mg/mL500 mcg80 units
2 mL25 mg/mL250 mcgover syringe capacity
3 mL16.67 mg/mL166.7 mcgover syringe capacity
5 mL10 mg/mL100 mcgover syringe capacity

SS-31 units to draw at 2 mL

Reading a 50 mg vial reconstituted with 2 mL of bacteriostatic water on a U-100 insulin syringe.

DoseVolumeU-100 unitsDoses per vial
20 mg0.8 mL80 units2
40 mg1.6 mLover syringe capacity1
60 mg2.4 mLover syringe capacity0
80 mg3.2 mLover syringe capacity0
  • One dose is 80% of the whole vial — check the dose unit (mcg vs mg) before drawing.

Mixing and storage notes

  • Aim the water down the inside wall of the vial, never straight onto the powder, and let it dissolve without shaking.
  • Keep lyophilized vials at 2–8 °C and out of light; reconstituted vials are refrigerated and commonly discarded after about 28 days.
  • Full walkthrough: how to reconstitute peptides step by step.

SS-31 reconstitution questions

How do you reconstitute SS-31?

A common starting point for a 50 mg vial of SS-31 is 2 mL of bacteriostatic water, which gives 25 mg per mL. Aim the water down the inside wall of the vial, let it dissolve on its own, and roll gently instead of shaking. Pep-Tidy's reconstitution calculator will recalculate the numbers for any other vial size or water volume you use.

How many units of SS-31 is one dose?

At 25 mg per mL, that works out to about 160 units on a U-100 insulin syringe for a 40000 mcg dose. Change the vial size, water volume, or target dose in the calculator and the syringe reading updates with it. This is arithmetic, not a dosing recommendation.

What dosing schedule is reported for SS-31?

Published and community sources describe 40 mg (trial dose), subcutaneous, every day, typically run 24 weeks on. These figures describe what has been reported, not what anyone should take — talk to a licensed clinician first.

How should SS-31 be stored?

Lyophilized SS-31 vials are generally kept at 2–8 °C and out of light. Once reconstituted with bacteriostatic water they are refrigerated and commonly discarded after about 28 days. Never re-freeze a reconstituted vial and never shake it.