Semaglutide Reconstitution Calculator
The numbers below are pre-computed for a 5 mg vial of Semaglutide. Doses reported in the literature and in research protocols for Semaglutide sit around 0.25 → 2.4 mg, subcutaneous. Pep-Tidy does the arithmetic only — it is not medical or dosing advice.
How much water to add to a 5 mg Semaglutide vial
| Bacteriostatic water | Concentration | Per U-100 unit | Units for 250 mcg |
|---|---|---|---|
| 1 mL | 5 mg/mL | 50 mcg | 5 units |
| 2 mL | 2.5 mg/mL | 25 mcg | 10 units |
| 3 mL | 1.67 mg/mL | 16.7 mcg | 15 units |
| 5 mL | 1 mg/mL | 10 mcg | 25 units |
Semaglutide units to draw at 2 mL
Reading a 5 mg vial reconstituted with 2 mL of bacteriostatic water on a U-100 insulin syringe.
| Dose | Volume | U-100 units | Doses per vial |
|---|---|---|---|
| 125 mcg | 0.05 mL | 5 units | 40 |
| 250 mcg | 0.1 mL | 10 units | 20 |
| 375 mcg | 0.15 mL | 15 units | 13 |
| 500 mcg | 0.2 mL | 20 units | 10 |
Mixing and storage notes
- Aim the water down the inside wall of the vial, never straight onto the powder, and let it dissolve without shaking.
- Keep lyophilized vials at 2–8 °C and out of light; reconstituted vials are refrigerated and commonly discarded after about 28 days.
- Full walkthrough: how to reconstitute peptides step by step.
Semaglutide reconstitution questions
How do you reconstitute Semaglutide?
A common starting point for a 5 mg vial of Semaglutide is 2 mL of bacteriostatic water, which gives 2.5 mg per mL. Aim the water down the inside wall of the vial, let it dissolve on its own, and roll gently instead of shaking. Pep-Tidy's reconstitution calculator will recalculate the numbers for any other vial size or water volume you use.
How many units of Semaglutide is one dose?
At 2.5 mg per mL, that works out to about 10 units on a U-100 insulin syringe for a 250 mcg dose. Change the vial size, water volume, or target dose in the calculator and the syringe reading updates with it. This is arithmetic, not a dosing recommendation.
What dosing schedule is reported for Semaglutide?
Published and community sources describe 0.25 → 2.4 mg, subcutaneous, once weekly, typically run 16 weeks on. These figures describe what has been reported, not what anyone should take — talk to a licensed clinician first.
How should Semaglutide be stored?
Lyophilized Semaglutide vials are generally kept at 2–8 °C and out of light. Once reconstituted with bacteriostatic water they are refrigerated and commonly discarded after about 28 days. Never re-freeze a reconstituted vial and never shake it.