NAD+ Reconstitution Calculator
The numbers below are pre-computed for a 500 mg vial of NAD+. Doses reported in the literature and in research protocols for NAD+ sit around 50–100 mg, subcutaneous / im. Pep-Tidy does the arithmetic only — it is not medical or dosing advice.
How much water to add to a 500 mg NAD+ vial
| Bacteriostatic water | Concentration | Per U-100 unit | Units for 75 mg |
|---|---|---|---|
| 1 mL | 500 mg/mL | 5 mg | 15 units |
| 2 mL | 250 mg/mL | 2.5 mg | 30 units |
| 3 mL | 166.67 mg/mL | 1.667 mg | 45 units |
| 5 mL | 100 mg/mL | 1 mg | 75 units |
NAD+ units to draw at 10 mL
Reading a 500 mg vial reconstituted with 10 mL of bacteriostatic water on a U-100 insulin syringe.
| Dose | Volume | U-100 units | Doses per vial |
|---|---|---|---|
| 37.5 mg | 0.75 mL | 75 units | 13 |
| 75 mg | 1.5 mL | over syringe capacity | 6 |
| 112.5 mg | 2.25 mL | over syringe capacity | 4 |
| 150 mg | 3 mL | over syringe capacity | 3 |
Mixing and storage notes
- Aim the water down the inside wall of the vial, never straight onto the powder, and let it dissolve without shaking.
- Keep lyophilized vials at 2–8 °C and out of light; reconstituted vials are refrigerated and commonly discarded after about 28 days.
- Full walkthrough: how to reconstitute peptides step by step.
NAD+ reconstitution questions
How do you reconstitute NAD+?
A common starting point for a 500 mg vial of NAD+ is 10 mL of bacteriostatic water, which gives 50 mg per mL. Aim the water down the inside wall of the vial, let it dissolve on its own, and roll gently instead of shaking. Pep-Tidy's reconstitution calculator will recalculate the numbers for any other vial size or water volume you use.
How many units of NAD+ is one dose?
At 50 mg per mL, that works out to about 150 units on a U-100 insulin syringe for a 75000 mcg dose. Change the vial size, water volume, or target dose in the calculator and the syringe reading updates with it. This is arithmetic, not a dosing recommendation.
What dosing schedule is reported for NAD+?
Published and community sources describe 50–100 mg, subcutaneous / im, every other day, typically run 4 weeks on and 2 weeks off. These figures describe what has been reported, not what anyone should take — talk to a licensed clinician first.
How should NAD+ be stored?
Lyophilized NAD+ vials are generally kept at 2–8 °C and out of light. Once reconstituted with bacteriostatic water they are refrigerated and commonly discarded after about 28 days. Never re-freeze a reconstituted vial and never shake it.