LL-37 Reconstitution Calculator
The numbers below are pre-computed for a 5 mg vial of LL-37. Doses reported in the literature and in research protocols for LL-37 sit around 100–500 mcg, subcutaneous. Pep-Tidy does the arithmetic only — it is not medical or dosing advice.
How much water to add to a 5 mg LL-37 vial
| Bacteriostatic water | Concentration | Per U-100 unit | Units for 100 mcg |
|---|---|---|---|
| 1 mL | 5 mg/mL | 50 mcg | 2 units |
| 2 mL | 2.5 mg/mL | 25 mcg | 4 units |
| 3 mL | 1.67 mg/mL | 16.7 mcg | 6 units |
| 5 mL | 1 mg/mL | 10 mcg | 10 units |
LL-37 units to draw at 2 mL
Reading a 5 mg vial reconstituted with 2 mL of bacteriostatic water on a U-100 insulin syringe.
| Dose | Volume | U-100 units | Doses per vial |
|---|---|---|---|
| 50 mcg | 0.02 mL | 2 units | 100 |
| 100 mcg | 0.04 mL | 4 units | 50 |
| 150 mcg | 0.06 mL | 6 units | 33 |
| 200 mcg | 0.08 mL | 8 units | 25 |
Mixing and storage notes
- Aim the water down the inside wall of the vial, never straight onto the powder, and let it dissolve without shaking.
- Keep lyophilized vials at 2–8 °C and out of light; reconstituted vials are refrigerated and commonly discarded after about 28 days.
- Full walkthrough: how to reconstitute peptides step by step.
LL-37 reconstitution questions
How do you reconstitute LL-37?
A common starting point for a 5 mg vial of LL-37 is 2 mL of bacteriostatic water, which gives 2.5 mg per mL. Aim the water down the inside wall of the vial, let it dissolve on its own, and roll gently instead of shaking. Pep-Tidy's reconstitution calculator will recalculate the numbers for any other vial size or water volume you use.
How many units of LL-37 is one dose?
At 2.5 mg per mL, that works out to about 4 units on a U-100 insulin syringe for a 100 mcg dose. Change the vial size, water volume, or target dose in the calculator and the syringe reading updates with it. This is arithmetic, not a dosing recommendation.
What dosing schedule is reported for LL-37?
Published and community sources describe 100–500 mcg, subcutaneous, every other day, typically run 4 weeks on and 4 weeks off. These figures describe what has been reported, not what anyone should take — talk to a licensed clinician first.
How should LL-37 be stored?
Lyophilized LL-37 vials are generally kept at 2–8 °C and out of light. Once reconstituted with bacteriostatic water they are refrigerated and commonly discarded after about 28 days. Never re-freeze a reconstituted vial and never shake it.